Frederick Douglass

Frederick Douglass Matthew Peachey Immunology 3/4/02 Toolbox #5Immunoprecipitation and Use of Antibodies in Isolation of Genes One of the most useful discoveries of recent apply science is the ability to isolate individual genes from an organisms stallion genome and then locate that gene. There be several(prenominal) methods on tap(predicate) to achieve this goal, many of which make use of antibodies to identify potions of molecules. For proteins such(prenominal) as cell surface proteins, it is very difficult to purify a protein solution.

To make antibodies to these casings of proteins, whole cells or cell mixtures are injected into rabbits and the antibodi es later collected. The antibodies must be separated from the other causas in sera however. To accomplish this, techniques such as affinity chromatography are utilise to isolate antibodies form sera. Isolated antibodies can then be added to a protein solution, allowing the binding of particular proteins causing precipitation from solution. This type ...If you want to get a full essay, order it on our website: OrderEssay.net

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